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Conventional amplicon-based methods for NGS Library Prep target enrichment involve two PCR steps: one to amplify the regions of interest, followed by a second PCR reaction to add adapter sequences (indexes) for NGS. While these methods are well established there is always a high risk of cross contamination or carry-over from first to second rounds of amplification.
RC-PCR eliminates risk of cross-contamination and pipetting error
Reverse Complement PCR (RC-PCR) is the only single step NGS Library Prep method as simple as a single PCR, combining indexing and PCR amplification in a single tube and reaction. NGS Library Prep will never be any simpler and safer. See the video animation of the working principle below.
RC-PCR is IP protected.
US Patent / WO Patent / EP Patent
Exclusive license granted to NimaGen B.V., Nijmegen, The Netherlands by SALISBURY NHS FOUNDATION TRUST (SALISBURY, WILTSHIRE, GB)
It is strictly prohibited to use, produce, distribute, import, or sell RC-PCR based methods or kits without the patent owner's written consent.
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