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BBS_Nimagen

EasySeq™ NGS Library Prep
by Reverse Complement PCR

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NGS Library Prep Contamination Concerns?

Conventional amplicon-based methods for NGS Library Prep target enrichment involve two PCR steps: one to amplify the regions of interest, followed by a second PCR reaction to add adapter sequences (indexes) for NGS. While these methods are well established there is always a high risk of cross contamination or carry-over from first to second rounds of amplification.

RC-PCR eliminates risk of cross-contamination and pipetting error

Reverse Complement PCR (RC-PCR) is the only single step NGS Library Prep method as simple as a single PCR, combining indexing and PCR amplification in a single tube and reaction. NGS Library Prep will never be any simpler and safer. See the video animation of the working principle below.

RC-PCR is IP protected.

US Patent / WO Patent / EP Patent

Exclusive license granted to NimaGen B.V., Nijmegen, The Netherlands by SALISBURY NHS FOUNDATION TRUST (SALISBURY, WILTSHIRE, GB)

It is strictly prohibited to use, produce, distribute, import, or sell RC-PCR based methods or kits without the patent owner's written consent.

EasySeq™ NGS Library Prep by RC-PCR Products

NGSG

EasySeq™ 18S and Cyp51A rRNA Fungal ID Kit, 96 rxn

NGSR

EasySeq™ PALB2 Sequencing Kit, 96 rxn

NGSR

EasySeq™ CFTR HotSpot Sequencing Kit, 96 rxn

NGSR

EasySeq™ CFTR Sequencing Kit, 96 rxn

NGSR

EasySeq™ BRCA1/2 + CHEK2 HS Sequencing Kit, 96 rxn

mYSTR

IDseek® mYSTR™ Y-Chromosomal STR Profiling Kit, 96 rxn

OmniSTR

IDseek® OmniSTR™ Global Autosomal STR Profiling Kit, 96 rxn

idSeek_Mito

IDseek® Mitochondrial DNA HVR Sequencing by MPS Kit, 96 rxn

Mito_FG

EasySeq™ BRCA1/2 + CHEK2 HS Sequencing Kit, 96 rxn

idSeek_Omni_SNP

IDseek® OmniSNP™ Identity Informative SNP Typing Kit, 96 rxn

NGSR

EasySeq™ TP53 Sequencing Kit, 96 rxn

AmpliClean-totaal

AmpliClean™ Cleanup Kit, Magnetic Beads (5 mL)

AmpliClean-totaal

AmpliClean™ Cleanup Kit, Magnetic Beads (50 mL)

AmpliClean-totaal

AmpliClean™ Cleanup Kit, Magnetic Beads (500 mL)

SARS_COV2

EasySeq™ SARS-CoV-2 WGS Library Prep Kit, 96 rxn

SARS_COV2

EasySeq™ SARS-CoV-2 WGS Library Prep Kit, 384 rxn

NGSR

EasySeq™ Human WES WGS Sample Tracking Kit, 96 rxn

NGSR

EasySeq™ 16S rRNA Bacterial ID Kit, 96 rxn

For further details click here

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